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- Title
Salmonella Typhimurium biofilm disruption by a human antibody that binds a pan-amyloid epitope on curli.
- Authors
Tursi, Sarah A.; Puligedda, Rama Devudu; Szabo, Paul; Nicastro, Lauren K.; Miller, Amanda L.; Qiu, Connie; Gallucci, Stefania; Relkin, Norman R.; Buttaro, Bettina A.; Dessain, Scott K.; Tükel, Çagla
- Abstract
Bacterial biofilms, especially those associated with implanted medical devices, are difficult to eradicate. Curli amyloid fibers are important components of the biofilms formed by the Enterobacteriaceae family. Here, we show that a human monoclonal antibody with pan-amyloid-binding activity (mAb 3H3) can disrupt biofilms formed by Salmonella enterica serovar Typhimurium in vitro and in vivo. The antibody disrupts the biofilm structure, enhancing biofilm eradication by antibiotics and immune cells. In mice, 3H3 injections allow antibiotic-mediated clearance of catheter-associated S. Typhimurium biofilms. Thus, monoclonal antibodies that bind a pan-amyloid epitope have potential to prevent or eradicate bacterial biofilms. Curli amyloid fibers are important components of bacterial biofilms formed by E. coli and Salmonella. Here, Tursi et al. show that a human monoclonal antibody with pan-amyloid binding activity can disrupt biofilms formed by Salmonella Typhimurium in vitro and in vivo.
- Subjects
SALMONELLA typhimurium; SALMONELLA enterica serovar typhimurium; MONOCLONAL antibodies; ARTIFICIAL implants
- Publication
Nature Communications, 2020, Vol 11, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-020-14685-3