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- Title
Cannabidiol Suppresses Angiogenesis and Stemness of Breast Cancer Cells by Downregulation of Hypoxia-Inducible Factors-1α.
- Authors
Jo, Min Jee; Kim, Bu Gyeom; Kim, Woo Young; Lee, Dae-Hee; Yun, Hye Kyeong; Jeong, Soyeon; Park, Seong Hye; Kim, Bo Ram; Kim, Jung Lim; Kim, Dae Yeong; Lee, Sun Il; Oh, Sang Cheul
- Abstract
Simple Summary: Cannabidiol (CBD), one of the compounds present in the marijuana plant, has antitumor properties. However, the effect of CBD on breast cancer remains unclear. The aim of this study was to assess the effects of CBD for the angiogenesis and stemness of breast cancer cells by decreasing the expression of hypoxia-induced factor-1α (HIF-1α) through the Src/von Hippel–Lindau tumor suppressor protein (VHL) interaction. CBD can suppress angiogenesis and stem cell-like properties of breast cancer through Src/VHL/HIF-1α signaling. To assess the effect of Cannabidiol (CBD) on the angiogenesis and stemness of breast cancer cells as well as proliferation. Methods: mRNA level and the amount of protein of vascular endothelial growth factor (VEGF) were determined by qRT-PCR and ELISA. The angiogenic potential of breast cancer cells under hypoxic conditions was identified by the HUVEC tube formation assay. The degradation of HIF-1α by CBD and the Src/von Hippel–Lindau tumor suppressor protein (VHL) interaction were assessed by a co-immunoprecipitation assay and Western blotting. To identify the stemness of mamospheres, they were evaluated by the sphere-forming assay and flow cytometry. Results: CBD can suppress angiogenesis and stem cell-like properties of breast cancer through Src/VHL/HIF-1α signaling. CBD may potentially be utilized in the treatment of refractory or recurrent breast cancer.
- Subjects
CANNABIDIOL; PROTEINS; REVERSE transcriptase polymerase chain reaction; FLOW cytometry; VON Hippel-Lindau disease; WESTERN immunoblotting; PRECIPITIN tests; CELLULAR signal transduction; PATHOLOGIC neovascularization; STEM cells; MESSENGER RNA; ENZYME-linked immunosorbent assay; CELL lines; MOLECULAR structure; VASCULAR endothelial growth factors; POLYMERASE chain reaction; BREAST tumors
- Publication
Cancers, 2021, Vol 13, Issue 22, p5667
- ISSN
2072-6694
- Publication type
Article
- DOI
10.3390/cancers13225667