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- Title
Simple and Sensitive Technique for Fentanyl Quantification in Urine and Plasma by GC-MS.
- Authors
Sharma, Priyamvada; Venkatesh, Bharat; Murthy, Pratima; Sharma, Manoj Kumar
- Abstract
This work reports a sensitive and specific gas chromatography (GC) mass spectrometry (MS) method for the detection of subnanogram concentrations of fentanyl in plasma. Objective- Fentanyl is a high-potency, rapid–onset synthetic opioid prescribed for the treatment of chronic pain and used as a surgical anesthetic. In the past decade its abuse and accidental overdose have steadily been on the rise. Method- This study was planned to develop an analytical procedure for fentanyl detection in biological samples which will help in to distinguish between therapeutic drug use and abuse. For sample preparation Solid phase extraction coupled (SPE) was opted while quantification was done with Gas Chromatograph Mass Spectrometer (GC-MS). GC-MS is among the best known precise and effective techniques for biological samples analysis. Chromatographic separation was achieved on using DB-5MS column on a selected ion monitoring (SIM) mode. Results-Signal to noise ratio was 3:1.The linearity lay between 0.62-40ng/ml correlation coefficient was ≥ 0.9899 and recovery was ≥80%. Limit of Detection was 0.6ng/ml and limit of quantification was 0.24ng/ml. Conclusion- Current analytical procedure for fentanyl quantification had good specificity and sensitivity and showed good recovery. This method will be helpful for quantification of fentanyl in clinical and can be applied to forensic as well .
- Subjects
FENTANYL; SOLID phase extraction; MASS spectrometry; MASS spectrometers; CHEMICAL sample preparation; GAS chromatography
- Publication
Indian Journal of Forensic Medicine & Toxicology, 2019, Vol 13, Issue 3, p78
- ISSN
0973-9122
- Publication type
Article
- DOI
10.5958/0973-9130.2019.00171.3