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- Title
Augmented brain 5-HT crosses the blood–brain barrier through the 5-HT transporter in rat.
- Authors
Nakatani, Yasushi; Sato‐Suzuki, Ikuko; Tsujino, Naohisa; Nakasato, Akane; Seki, Yoshinari; Fumoto, Masaki; Arita, Hideho
- Abstract
The present study re-evaluated an existing notion that serotonin (5-hydroxytryptamine; 5-HT) could not cross the brain to the circulating blood via the blood–brain barrier (BBB). To elevate brain 5-HT alone, 5-hydroxytryptophan (5-HTP; 30–75 mg/kg) was administrated intravenously to anaesthetized rats that had undergone gastrointestinal and kidney resections along with liver inactivation (organs contributing to increasing blood 5-HT after 5-HTP administration). A microdialysis method and HPLC system were used to determine the brain 5-HT levels in samples collected from the frontal cortex. Blood 5-HT levels were determined from whole blood, not platelet-poor plasma, collected from the central vein. We found that blood 5-HT levels showed a significant augmentation whenever brain 5-HT levels were significantly elevated after the administration of 5-HTP in those rats with the abdominal surgical procedures. This elevation was abolished after pretreatment with a selective serotonin reuptake inhibitor (fluoxetine; 10 mg/kg i.v.), although brain 5-HT levels remained augmented. These results indicate that augmented brain 5-HT can cross the BBB through the 5-HT transporter from the brain to the circulating blood.
- Subjects
BLOOD circulation disorders; MICRODIALYSIS; HYDROXYTRYPTOPHAN; GASTROINTESTINAL diseases; SEROTONIN; FLUOXETINE
- Publication
European Journal of Neuroscience, 2008, Vol 27, Issue 9, p2466
- ISSN
0953-816X
- Publication type
Article
- DOI
10.1111/j.1460-9568.2008.06201.x