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- Title
Effects of Prostaglandin E<sub>1</sub> on the Preparation of Platelet Concentrates in Dogs.
- Authors
Segawa, K.; Kondo, T.; Kimura, S.; Fujimoto, A.; Kato, T.; Ishikawa, T.; Neo, S.; Hisasue, M.; Yamada, T.; Tsuchiya, R.
- Abstract
Background Platelet concentrates ( PC) are prepared by centrifugation of platelet-rich plasma ( PRP) that is prepared by centrifugation of whole blood. The resuspension of the platelet pellet during PC preparation from dogs is difficult because of platelet activation induced by centrifugation. Objectives To investigate the efficacy of adding prostaglandin E1 ( PGE1) to prevent platelet activation during PC preparation from dogs. Animals Fifteen healthy Beagle dogs. Methods Prospective, experimental trial: PGE1 was added to PRP before the high-speed centrifugation during PC preparation. To estimate the effect of this addition, we assessed the platelet aggregability before transfusion, the survival of the platelets after transfusion, and the platelet reactivity after transfusion, which is estimated by the P-selectin expression of the platelets when stimulated by thrombin. Results The difficulty associated with platelet resuspension was resolved by PGE1. PGE1 strongly inhibited platelet aggregation induced by collagen and ADP; however, it recovered after the platelets were resuspended in plasma without PGE1 (mean aggregation ratio; collagen: 10.00-80.80%, ADP: 8.20-53.60%). Survival of the platelets after transfusion was not affected by PGE1 (mean 8.04 and 7.56 days, without and with PGE1), and thrombin-induced P-selectin expression after transfusion in PGE1-treated PC was also well maintained (mean positive ratio 53.7 and 47.9%, before and 24 hours after transfusion). Conclusions and Clinical Importance The addition of PGE1 in PRP before the centrifugation of PRP can improve the preparation efficiency of PC from dogs, while maintaining the therapeutic efficacy of the platelets.
- Subjects
DOG diseases; PROSTAGLANDINS E; SELECTINS; CELL adhesion molecules; ANIMAL diseases
- Publication
Journal of Veterinary Internal Medicine, 2012, Vol 26, Issue 2, p370
- ISSN
0891-6640
- Publication type
Article
- DOI
10.1111/j.1939-1676.2011.00881.x