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- Title
Assessing Almond and Peanut Allergens Using Commercially Available Immunoanalytical Kits and LC-MS/MS: A Case Study.
- Authors
DALY, MATTHEW; ANSARI, PARISA; HÄUBL, GEORG; ROGERS, ADRIAN; BRUNNER, KURT
- Abstract
With an ever-increasing allergic population and an emerging market for allergen-free foods, accurate detection of allergens in foods has never been more important. Although ELISA-based methods are the most widely used for detection of allergens in food, there is a need for the development of orthogonal approaches. A commercial ELISA detected a relatively high concentration of peanut and almond in an allergen-free product. However, another commercial ELISA declared a low peanut concentration and was negative for almond. Further testing using a commercial almond lateral-flow device confirmed the results from the second ELISA kit and demonstrated that the positive detection of almond was due to cross-reactivity. An MS method was used for final confirmation that the reported results were negative for both almond and peanut.
- Subjects
FOOD allergy; ALLERGENS; ENZYME-linked immunosorbent assay; NUT allergy; ALMOND
- Publication
Journal of AOAC International, 2018, Vol 101, Issue 1, p96
- ISSN
1060-3271
- Publication type
Article
- DOI
10.5740/jaoacint.17-0398