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- Title
Exosomes derived from miR-338-3p-modified adipose stem cells inhibited inflammation injury of chondrocytes via targeting RUNX2 in osteoarthritis.
- Authors
Li, ChunLiang; Li, Wei; Pu, GengZang; Wu, JingWen; Qin, Feng
- Abstract
Background: Osteoarthritis (OA) is a chronic degenerative disease that is one of the main causes of disability in middle-aged and elderly people. Adipose stem cell (ASC)-derived exosomes (ASC-Exo) could repair cartilage damage and treat OA. MiRNA-338-3p expression was confirmed to play a role in inhibiting proinflammatory cytokines. Herein, we aimed to explore the mechanism by which exosomes derived from miR-338-3p overexpressing ASCs protects chondrocytes from interleukin (IL)-1β-induced chondrocyte change. Methods: Exosomes were extracted from ASCs transfected with miR-338-3p or its antisense inhibitor. The ASC-Exos (miR-338-3p silencing/overexpression) were incubated with IL-1β-induced ATDC5 cells, followed by evaluation of the chondrocyte proliferation, degradation, and inflammation injury. Results: In vitro results revealed that ASC-Exos inhibited the expression of prostaglandin E2 (PGE2), IL-6, IL-1β, and TNF-α, as well as promoted the proliferation of ATDC5 cells. Moreover, ASC-Exos inhibited inflammation injury and degradation of ATDC5 cells by transferring miR-338-3p. Luciferase reporter assays showed that RUNX2 was a target gene of miR-338-3p. Additionally, RUNX2 overexpression in ATDC5 cells reversed the protective effect of miR-338-3p on chondrocytes. Taken together, this study demonstrated that exosomes secreted from miR-338-3p-modified ASCs were effective in the repair of IL-1β-induced chondrocyte change by inhibiting RUNX2 expression. Conclusions: Our result provided valuable data for understanding the mechanism of ASC-Exos in OA treatment.
- Subjects
INFLAMMATION prevention; CARTILAGE cells; IN vitro studies; PROSTAGLANDINS E; INTERLEUKINS; EXOSOMES; CHONDROGENESIS; ANTI-inflammatory agents; ANIMAL experimentation; REGENERATION (Biology); ONE-way analysis of variance; MICRORNA; INTERLEUKIN-1; GENE expression; FAT cells; OSTEOARTHRITIS; CELL proliferation; TUMOR necrosis factors; DESCRIPTIVE statistics; TRANSCRIPTION factors; DATA analysis software; MESENCHYMAL stem cells; MICE; PHARMACODYNAMICS
- Publication
Journal of Orthopaedic Surgery & Research, 2022, Vol 17, Issue 1, p1
- ISSN
1749-799X
- Publication type
Article
- DOI
10.1186/s13018-022-03437-2