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- Title
Transcriptional regulation of the human GM3 synthase (hST3Gal V) gene during monocytic differentiation of HL-60 cells
- Authors
Choi, Hee-Jung; Chung, Tae-Wook; Kang, Nam-Young; Kim, Kyoung-Sook; Lee, Young-Choon; Kim, Cheorl-Ho
- Abstract
We studied the transcriptional regulation of human GM3 synthase (hST3Gal V) during monocytic differentiation of HL-60 cells induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). Northern blot and reverse transcription polymerase chain reaction indicated that the induction of hST3Gal V by TPA is regulated at the transcriptional level. To elucidate the mechanism underlying the regulation of hST3Gal V gene expression during the differentiation of HL-60 cells induced by TPA, we characterized the promoter region of the hST3Gal V gene. Functional analysis of the 5′-flanking region of the hST3Gal V gene by the transient expression method showed that the −177 to −83 region, which contains a cAMP responsive element binding protein (CREB) binding site at −143, functions as the TPA-inducible promoter in HL-60 cells. In addition, gel shift assays and site-directed mutagenesis indicated that the CREB binding site at −143 is crucial for the TPA-induced expression of the hST3Gal V in HL-60 cells.
- Subjects
TRANSCRIPTION factors; CELLS; ACETATES; POLYMERASE chain reaction
- Publication
FEBS Letters, 2003, Vol 555, Issue 2, p204
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(03)01227-4