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- Title
Identification of neuropeptide FF-related peptides in human cerebrospinal fluid by mass spectrometry
- Authors
Burlet-Schiltz, Odile; Mazarguil, Honoré; Sol, Jean-Christophe; Chaynes, Patrick; Monsarrat, Bernard; Zajac, Jean-Marie; Roussin, Anne
- Abstract
Several neuropeptide FF (NPFF)-related peptides, known as modulators of the opioid system, have been previously characterized in bovine and rodent brain. Reverse-phase high pressure liquid chromatography (HPLC) fractions of a human with normal pressure hydrocephalus cerebrospinal fluid (CSF), co-migrating with NPFF-related synthetic peptides, were characterized by capillary HPLC coupled on-line to nanospray ion trap tandem mass spectrometry. Two peptides present in the pro-NPFFA precursor, NPAF (AGEGLNSQFWSLAAPQRF-NH2) and NPSF (SLAAPQRF-NH2), were identified. The monitoring of NPFF-related peptides in human CSF can be helpful to understand their roles in pain sensitivity.
- Subjects
NEUROPEPTIDES; CEREBROSPINAL fluid
- Publication
FEBS Letters, 2002, Vol 532, Issue 3, p313
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)03686-4