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- Title
BcPMI2, isolated from non-heading Chinese cabbage encoding phosphomannose isomerase, improves stress tolerance in transgenic tobacco.
- Authors
Wang, Xuehua; Zhang, Shuo; Hu, Die; Zhao, Xiaojun; Li, Yan; Liu, Tongkun; Wang, Jianjun; Hou, Xilin; Li, Ying
- Abstract
Phosphomannose isomerase (PMI) is an enzyme that catalyses the first step of the l-galactose pathway for ascorbic acid (AsA) biosynthesis in plants. To clarify the physiological roles of PMI in AsA biosynthesis, the cDNA sequence of PMI was cloned from non-heading Chinese cabbage ( Brassica campestris ssp. chinensis Makino) and overexpressed in tobacco transformed with Agrobacterium tumefaciens. The AsA and soluble sugar contents were lower in 35S:: BcPMI2 tobacco than in wild-type tobacco. However, the AsA level in BcPMI2-overexpressing plants under stress was significantly increased. The T seed germination rate of transgenic plants was higher than that of wild-type plants under NaCl or HO treatment. Meanwhile, transgenic plants showed higher tolerance than wild-type plants. This finding implied that BcPMI2 overexpression improved AsA biosynthetic capability and accumulation, and evidently enhanced tolerance to oxidative and salt stress, although the AsA level was lower in transgenic tobacco than in wild-type tobacco under normal condition.
- Subjects
CHINESE cabbage; BIOCHEMICAL engineering; PLANT genetic engineering; AGROBACTERIUM; TRANSGENIC organisms
- Publication
Molecular Biology Reports, 2014, Vol 41, Issue 4, p2207
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-014-3072-2