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- Title
Regulation of Gene Expression by Low Levels of Ultraviolet-B Radiation in Pisum sativum: Isolation of Novel Genes by Suppression Subtractive Hybridisation.
- Authors
Sävenstrand, Helena; Brosché, Mikael; Strid, Åke
- Abstract
Suppression subtractive hybridisation was used to isolate genes differentially regulated by low levels (UV-BBE,300 0.13 W m–2) of ultraviolet-B radiation (UV-B; 290–320 nm) in Pisum sativum. Six genes were regulated, two of which were novel. The mRNA levels for these two (PsTSDC and PsUOS1) were increased and depressed by UV-B treatment, respectively. Domains in the PsTSDC translation product was similar to TIR (Toll-Interleukin-1 receptor-similar) domains and a NB-ARC domain (nucleotide-binding domain in APAF-1, R gene products and CED-4). The PsUOS1 translation product was similar to an open reading frame in Arabidopsis. Genes encoding embryo-abundant protein (PsEMB) and S-adenosyl-l-methionine synthase (PsSAMS) were induced by UV-B, whereas the transcript levels for genes encoding sucrose transport protein (PsSUT) or ribulose-5-phosphate 3-epimerase (PsR5P3E) were decreased. These regulation patterns are novel, and the PsEMB and PsR5P3E sequences are reported for the first time. The stress-specificity of regulation of these genes were tested by ozone fumigation (100 ppb O3). Qualitatively, the similarity of expression after both UV-B and ozone exposure suggests that, for these genes, similar stress-response pathways are in action.
- Subjects
PEA genetics; GENE expression in plants; GENETIC regulation in plants; EFFECT of ultraviolet radiation on plants; PLANT hybridization
- Publication
Plant & Cell Physiology, 2002, Vol 43, Issue 4, p402
- ISSN
0032-0781
- Publication type
Article