We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Regulation of Transient Receptor Potential Melastatin 7 (TRPM7) Currents by Mitochondria.
- Authors
Byung Joo Kim; Ju-Hong Jeon; Seon Jeong Kim; Insuk So; Ki Whan Kim
- Abstract
Mitochondria play a central role in energy-generating processes and may be involved in the regulation of channels and receptors. Here we investigated TRPM7, an ion channel and functional kinase, and its regulation by mitochondria. Proton ionophores such as CCCP elicited a rapid decrease in outward TRPM7 whole-cell currents but a slight increase in inward currents with pipette solutions containing no MgATP. With pipette solutions containing 3 mM MgATP, however, CCCP increased both outward and inward TRPM7 currents. This effect was reproducible and fully reversible, and repeated application of CCCP yielded similar decreases in current amplitude. Oligomycin, an inhibitor of F1/FOATP synthase, inhibited outward whole-cell currents but did not affect inward currents. The respiratory chain complex I inhibitor, rotenone, and complex III inhibitor, antimycin A, were without effect as were kaempferol, an activator of the mitochondrial Ca2+ uniporter, and ruthenium red, an inhibitor of the mitochondrial Ca2+ uniporter. These results suggest that the inner membrane potential (as regulated by proton ionophores) and the F1/FO-ATP synthase of mitochondria are important in regulating TRPM7 channels.
- Publication
Molecules & Cells (Springer Nature), 2007, Vol 23, Issue 3, p363
- ISSN
1016-8478
- Publication type
Article
- DOI
10.1016/s1016-8478(23)10727-8