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- Title
In Vivo Antitumor Effect of Herpes Simplex Virus Thymidine Kinase Gene Therapy in Rat Hepatocellular Carcinoma: Feasibility of Adenovirus-mediated Intra-arterial Gene Delivery.
- Authors
Hee-Chung Kwon; Jung-Hyeon Kim; Keun-Cheol Kim; Ki-Ho Lee; Jae-Hyun Lee; Byung Hee Lee; Kang-Hyun Lee; Ja-June Jang; Choon-Taek Lee; Hansoo Lee; Chang-Min Kim
- Abstract
Transfer of the herpes simplex virus-thymidine kinase gene, followed by the administration of ganciclovir (HSV-tk/GCV), has been a major approach for cancer gene therapy. We investigated the antitumor effect of the HSV-tk/GCV strategy with the rat orthotopic hepatocellular carcinoma (HCC) model and the tumor-selective gene delivery by an adenovirus-mediated gene transfer through the hepatic artery. The complete antitumor effect was demonstrated, after the treatment with GCV in rat HCC established by the Implantation of HSV-tk transferred rat HCC cells. The in vivo bystander effect was also observed. The marked infiltration of CD4+ and CD8+ T lymphocytes, macrophages and NK cells were found in the tumor area. After the injection of adenovirus carrying the LacZ gene into the hepatic artery, the selective expression of transgene in the tumor cell was achieved. These findings indicate that the HSV-tk/GCV strategy, using an adenoviral vector, could be a promising avenue for the treatment of hepatocellular carcinoma.
- Subjects
CANCER treatment; HERPES simplex virus; CANCER genetics; ANTIVIRAL agents; LIVER cancer; BLOOD vessels
- Publication
Molecules & Cells (Springer Nature), 2001, Vol 11, Issue 2, p170
- ISSN
1016-8478
- Publication type
Article
- DOI
10.1016/s1016-8478(23)17021-x