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- Title
Quantitative PCR detection of t(11;14) bcl-1/JH in mantle cell lymphoma patients: comparison of peripheral blood and bone marrow aspirate samples.
- Authors
DI MARTINO, S.; CATAPANO, O.; SIESTO, S. R.; DI PAOLO, M.; PUGLIESE, S.; MORELLI, C. D.; FIORICA, F.; VARRIALE, E.; DI FRANCIA, R.; ABBADESSA, A.
- Abstract
OBJECTIVE: Mantle cell lymphoma (MCL) is a non-Hodgkin lymphoma (NHL) featured by participation of the lymph nodes, spleen, blood and bone marrow with a short remission period to standard therapies and a median overall survival of 4-5 years. PATIENTS AND METHODS: In this study, we compare the levels of bcl-1/JH fusion products detected by q-PCR in the concurrent peripheral blood (PB) and bone marrow (BM) aspirate samples from 7 patients with MCL. RESULTS: In patients with moderate to high levels of bcl-1/JH copies, the results of q-PCR analysis of PB and BM aspirate samples correlate well. In patients with high levels of bcl-1/JH copies, instead, PB levels are a good indication of tumor burden. Finally, in patients with low levels of bcl-1/JH copies, the t(11;14) may be detected by identification of neoplastic cells. CONCLUSIONS: Our data suggest that PB can be reliably used in place of BM aspirate both for detection of translocation status during minimal residual disease monitoring and for a possible molecular relapse, especially in those patients who have moderate to high levels of bcl-1/JH copies. If these results will be confirmed on a wider number of MCL patients, future study will be required to address the issue.
- Subjects
MANTLE cell lymphoma; DIAGNOSTIC use of polymerase chain reaction; MOLECULAR diagnosis; CHROMOSOMAL translocation; TUMOR diagnosis
- Publication
European Review for Medical & Pharmacological Sciences, 2015, Vol 19, Issue 24, p4801
- ISSN
1128-3602
- Publication type
Article