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- Title
Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets.
- Authors
Won Gi Yoo; Sun-Min Kim; Eun Jeong Won; Ji-Yun Lee; Fuhong Dai; Ho Choon Woo; Ho-Woo Nam; Tae Im Kim; Jeong-Hee Han; Dongmi Kwak; Yun Sang Cho; Seung-Won Kang; Tong-Soo Kim; Xing-Quan Zhu; Chunren Wang; Heejeong Youn; Sung-Jong Hong
- Abstract
To investigate the prevalence of Toxoplasma gondii in pork on the market in Korea, an in-house enzyme-linked immunosorbent assay for tissue fluid (CAU-tf-ELISA) was developed using a soluble extract of T. gondii RH strain tachyzoites. As the standard positive controls, the piglets were experimentally infected with T. gondii: Group A (1,000 cysts-containing bradyzoites), Group B (500 cysts-containing bradyzoites) and Group C (1.0×103 or 1.0×104 tachyzoites). The CAU-tf-ELISA demonstrated infection intensity-dependent positivity toward tissue fluids with average cut-off value 0.15: 100% for Group A, 93.8% for Group B and 40.6% for Group C. When tissue-specific cut-off values 0.066- 0.199 were applied, CAU-tf-ELISA showed 96.7% sensitivity, 100% specificity, 100% positive and 90.0% negative predictive values. When compared with the same tissue fluids, performance of CAU-tf-ELISA was better than that of a commercial ELISA kit. Of the 583 Korea domestic pork samples tested, anti-T. gondii antibodies were detected from 9.1% of whole samples and 37.9% from skirt meat highest among pork parts. In the 386 imported frozen pork samples, 1.8% (skirt meat and shoulder blade) were positive for anti-T. gondii antibodies. In Korea, prevalence of anti-T. gondii antibodies in the pork on retail markets appeared high, suggesting that regulations on pig farming and facilities are necessary to supply safe pork on the tables.
- Subjects
TOXOPLASMA gondii; PORK industry; PIGLETS; ENZYME-linked immunosorbent assay; DISEASE prevalence
- Publication
Korean Journal of Parasitology, 2018, Vol 56, Issue 5, p437
- ISSN
0023-4001
- Publication type
Article
- DOI
10.3347/kjp.2018.56.5.437