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- Title
THE PRODUCTION OF LYMPHOCYTE MITOGENIC FACTOR AND MIGRATION-INHIBITION FACTOR BY ANTIGEN-STIMULATED LYMPHOCYTES OF SUBJECTS WITH GRASS POLLEN ALLERGY.
- Authors
Maini, R. N.; Dumonde, D. C.; Faux, J. A.; Hargreave, F. E.; Pepys, J.
- Abstract
Peripheral blood lymphocytes of twenty-three subjects with grass pollen allergy were cultured with grass pollen antigen for 3 days. After harvesting, the culture supernatants were added to fresh autologous lymphocytes which were maintained in culture for 6 days. Cellular uptake of [³H]thymidine was measured during the sixth day of culture, and revealed that the lymphocyte culture supernatants stimulated greater thymidine uptake than expected from the lymphocyte transformation response to corresponding amounts of antigen. The supernatant factor which mediated this effect was termed `lymphocyte mitogenic factor' by analogy with a similar response of lymphocytes in clinical and experimental delayed hypersensitivity. Lymphocyte culture supernatants were also tested for migration- inhibition factor by their ability to inhibit the migration of guinea-pig macrophages.
- Subjects
ALLERGIES; INTERLEUKIN-2; ANTIGENS; THYMIDINE; LYMPHOCYTES; MACROPHAGES
- Publication
Clinical & Experimental Immunology, 1971, Vol 9, Issue 4, p449
- ISSN
0009-9104
- Publication type
Article