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- Title
Patterns ofDwarfexpression and brassinosteroid accumulation in tomato reveal the importance of brassinosteroid synthesis during fruit development.
- Authors
Montoya, Teresa; Nomura, Takahito; Yokota, Takao; Farrar, Kerrie; Harrison, Kate; Jones, Jonathan G. D.; Kaneta, Tsuyoshi; Kamiya, Yuji; Szekeres, Miklos; Bishop, Gerard J.
- Abstract
Brassinosteroids (BRs) are essential for many physiological functions in plants, however little is known concerning where and when they are synthesized. This is especially true during flower and fruit production. To address this we have used a promoter-GUS reporter fusion and RT-PCR to determine the relative expression levels of the tomatoDwarf(D) gene that encodes a BR C-6 oxidase. In young seedlings GUS reporter activity was observed mainly in apical and root tissues undergoing expansion. In flowers GUS activity was observed in the pedicel joints and ovaries, whereas in fruits it was strongest during early seed development and was associated with the locular jelly and seeds. RT-PCR analysis showed that tissue-specific expression ofDwarfmRNA was consistent with that of theDwarf:GUS fusion. In good correlation with the high localDwarfactivity, quantitative measurements of endogenous BRs indicated intense biosynthesis in developing tomato fruits, which were also found to contain high amounts of brassinolide. Grafting experiments showed the lack of BR transport indicating that BR action occurs at the site of synthesis.
- Subjects
BRASSINOSTEROIDS; TOMATOES; FRUIT development; PLANT hormones; PLANTS; POLYMERASE chain reaction
- Publication
Plant Journal, 2005, Vol 42, Issue 2, p262
- ISSN
0960-7412
- Publication type
Article
- DOI
10.1111/j.1365-313X.2005.02376.x