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- Title
Analysis of Peripheral Blood Lymphocytes of AIDS and High-Risk Patients for Human Cytomegalovirus Transforming DNA Sequences.
- Authors
Razzaque, Abdur; Peters, Stephen M.; Gelmann, Edward P.; Sheridan, Michael J.; Rosenthal, Leonard J
- Abstract
Investigation into the presence of human cytomegalovirus (HCMV) transforming mtrll and mtrlΠ sequences in peripheral blood lymphocyte (PBL) specimens of AIDS and high-risk patients was carried out by nucleic acid hybridization analyses. These probes were selected because they were viral-specific and lacked homology to normal cellular DNAs. In Southern blot hybridizations carried out under stringent conditions, we detected HCMV mtrll sequences associated with the high-molecular-weight DNAs of PBLs in 17 of 37 patients either with AIDS/Kaposi's sarcoma or at high risk for AIDS. In comparison, only 2 of 17 DNA specimens from PBLs of healthy blood donors showed hybridization to mtrll sequences. The inability to detect hybridization to the mtrlΠ region in most mtrII-positive specimens suggested a specific retention of mtrll sequences. Our study suggests that the retention of mtrll sequences in high molecular weight DNA may constitute a risk factor for the development/ progression of AIDS. Alternatively, the retention of mtrll sequences may occur as a result of enhanced HCMVreplicationin patients with AIDS or at high risk for AIDS. Copyright © 1991 S. Karger AG, Basel
- Publication
Intervirology, 1991, Vol 32, Issue 1, p10
- ISSN
0300-5526
- Publication type
Article
- DOI
10.1159/000150180