We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Simple Sequence Repeat-Based Genetic Diversity and Analysis of Molecular Variance among on-Farm Native Potato Landraces from the Influence Zone of Camisea Gas Project, Northern Ayacucho, Peru.
- Authors
De la Cruz, Germán; Miranda, Tomás Yuret; Blas, Raúl Humberto; Neyra, Edgar; Orjeda, Gisella
- Abstract
The Andean native potato is an important food security crop and constitutes a gene pool for potato-breeding. To establish the current state of the native potatoes diversity in the influence zone of the Camisea Gas Project, North Ayacucho, Peru, landraces (n = 144) were collected. Three sampling populations (Anco, Chungui and Ticllas) were resolved using 10 simple sequence repeats (SSRs) markers that amplified 67 polymorphic alleles. Principal component, correspondence and cluster analysis revealed a minimum set of six SSRs to achieve DNA fingerprinting and cost-effective genetic diversity analysis. Analysis of molecular variance (AMOVA), average fixation index (FST = 0.279), and genetic distance index (SB) indicated that the genetic diversity of the native potatoes is high at the intra- and inter-population levels, and each of the three sampling populations constituted closed populations. The three populations were genetically distinct and contained unique genotypes and exclusive alleles. Misidentified native potatoes (10.41%) were found.
- Subjects
POTATO growing; MICROSATELLITE repeats; ANALYSIS of variance; DNA fingerprinting; FOOD crops; POTATOES; CHLOROPLAST DNA; GENETIC distance
- Publication
American Journal of Potato Research, 2020, Vol 97, Issue 2, p143
- ISSN
1099-209X
- Publication type
Article
- DOI
10.1007/s12230-020-09763-7