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- Title
Down-regulation of heme oxygenase-1 by hepatitis C virus infection in vivo and by the in vitro expression of hepatitis C core protein.
- Authors
Abdalla, Maher Y.; Britigan, Bradley E.; Feng Wen; Icardi, Michael; McCormick, Michael L.; LaBrecque, Douglas R.; Voigt, Michael; Brown, Kyle E.; Schmidt, Warren N.; Wen, Feng
- Abstract
Antioxidant enzymes, including heme oxygenase (HO)-1, are an important line of defense against oxidant-mediated liver injury. Because hepatitis C virus (HCV) infection appears to increase the production of oxidants, we evaluated levels of antioxidant enzymes and HO-1 in liver-biopsy samples from HCV-infected patients by immunoblot and semiquantitative reverse-transcriptase polymerase chain reaction. In HCV-infected liver samples, levels of immunoreactive HO-1 and HO-1 mRNA were >4-fold lower than levels in control samples, but levels of superoxide dismutase and catalase were unaffected. Immunohistochemical results confirmed the decreased expression of HO-1 in hepatocytes from liver samples from HCV-infected patients but not in those from patients with other chronic liver diseases. The expression of HO-1 was also reduced in cell lines that stably express HCV core protein, which suggests that core gene products are capable of regulating the expression of HO-1.
- Subjects
HEME oxygenase; HEPATITIS C virus; COMMUNICABLE diseases; ANTIOXIDANTS; INTERNAL medicine; MEDICAL research; PATHOLOGY; PROTEIN metabolism; RNA analysis; BIOCHEMISTRY; BIOPSY; CELL lines; COMPARATIVE studies; HEPATITIS viruses; IMMUNOHISTOCHEMISTRY; LIVER; PHENOMENOLOGY; RESEARCH methodology; MEDICAL cooperation; MEMBRANE proteins; OXIDOREDUCTASES; POLYMERASE chain reaction; RESEARCH; RNA; SUPEROXIDE dismutase; WESTERN immunoblotting; EVALUATION research; REVERSE transcriptase polymerase chain reaction; PHYSIOLOGY
- Publication
Journal of Infectious Diseases, 2004, Vol 190, Issue 6, p1109
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1086/423488