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- Title
A new method, distribution-analyzing latex immunoassay (DALIA), to measure specific immunoglobulin G against mite and wheat allergen in human sera.
- Authors
Xu, J. Z.; Matsuo, M.; Kamiyama, N.; Matsushita, H.; Sakai, Y.
- Abstract
A distribution-analyzing latex immunoassay (DALIA), based on the agglutination of latex particles coupled with mite or wheat allergen, was developed to determine allergen-specific IgG in human sera. The immune complex between chemically coupled-allergen latex and specific IgG was agglutinated specifically and efficiently by employing an IgM-type monoclonal second antibody with strong amplification activity The extent of agglutination was evaluated by determining the relative ratio of volumes (RV) of agglutinates to residual nonagglutinating particles with a particle counter. This method exhibited a high sensitivity (detection limit ⩽ 5 munits/ml) in the determination of allergen-specific IgG, and no influence of inhibitory factors such as competitive antibodies (specific-IgA-IgM) and nonspecific IgG (⩽ 320 mg ml) was observed The concentrations of specific IgG against mite allergen in the sera of 130 allergy patients with atopic dermatitis and 52 normal subjects were 22.3 ± 12.3 and 1 6.5 ± 4.2 units/mI, respectively, and the concentrations of specific IgG against wheat allergen in the same two groups were 5.4 ± 4.2 and 2.1 ± 2.2 units/ml, respectively. The coefficients of variation of intra- and interassay ranged from 3.4% to 11.2% in both cases. The present method is an excellent homogeneous immunoassay which may he used as a routine assay that can measure 50 samples per hour without prior treatment.
- Subjects
ALLERGENS; LATEX; MITES; IMMUNOGLOBULINS; WHEAT; ALLERGIES
- Publication
Allergy, 1994, Vol 49, Issue 5, p323
- ISSN
0105-4538
- Publication type
Article
- DOI
10.1111/j.1398-9995.1994.tb02276.x