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- Title
Universal protocol for generating 100bp size standard for endless usage.
- Authors
Natarajan, Chandrasekhar; Vijayan, Neetha N.; Vaidyan, Linda Koshy; Mathew, Anila; Srinivas, Lekshmy; Banerjee, Moinak
- Abstract
Developing countries are facing severe bottlenecks in the technological advancement in biotechnology, due to restrictions imposed by patent protected products and protocols. This calls for designing of simple and cost-effective alternatives for the indispensable products like DNA molecular weight markers. We demonstrate a novel, rapid and cost-effective method of making inhouse 100bp ladder for routine use. In our method we use a single forward primer and five reverse primers designed on the backbone sequence of a commonly used vector template. These primers are used at a universal annealing temperature to amplify ten DNA fragments of accurate size ranging from 100bp to 1000bp. Our PCR-based method can provide size standards for an endless usage.
- Subjects
LADDERS; COST effectiveness; POLYMERASE chain reaction; RAPD technique; BOTTLENECKS (Manufacturing); BIOTECHNOLOGY; MEDICAL protocols
- Publication
Electronic Journal of Biotechnology, 2008, Vol 11, Issue 2, p1
- ISSN
0717-3458
- Publication type
Article
- DOI
10.2225/vol11-issue2-fulltext-10