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- Title
MicroRNA Regulation for Inflammasomes in High Glucose‐Treated ARPE‐19 Cells.
- Authors
Kim, Ji Hong; Yu, Hyoseon; Kang, Ji Hye; Hong, Eun Hee; Kang, Min Ho; Seong, Mincheol; Cho, Heeyoon; Shin, Yong Un; Honda, Shigeru
- Abstract
Purpose. This study aimed to evaluate the expression of microRNAs (miRNAs) and inflammasomes in diabetes‐induced retinal cells and to determine their role in the pathogenesis of diabetic retinopathy (DR). Methods. To establish diabetes‐induced cell models, ARPE‐19 cells were treated with high glucose. The expression levels of five miRNAs (miR‐185, miR‐17, miR‐20a, miR‐15a, and miR‐15b) were measured in high glucose‐treated ARPE‐19 cells using real‐time quantitative polymerase chain reaction. Western blotting was performed to measure inflammasome expression in cellular models. miR‐17 was selected as the target miRNA, and inflammasome expression was measured following the transfection of an miR‐17 mimic into high glucose‐treated ARPE‐19 cells. Results. In high glucose‐treated ARPE‐19 cells, miRNA expression was substantially downregulated, whereas that of inflammasome components was significantly increased. Following the transfection of the miR‐17 mimic into high glucose‐treated ARPE‐19 cells, the levels of inflammasome components were significantly decreased. Conclusions. This study investigated the relationship between miRNAs and inflammasomes in diabetes‐induced cells using high glucose‐treated ARPE‐19 cells. These findings suggested that miR‐17 suppresses inflammasomes, thereby reducing the subsequent inflammatory response and indicating that miRNAs and inflammasomes could serve as new therapeutic targets for DR.
- Subjects
SOUTH Korea; PROTEINS; GLUCOSE; EPITHELIAL cells; RESEARCH funding; MICRORNA; DIABETIC retinopathy; POLYMERASE chain reaction; GENE expression; MICE; ANIMAL experimentation; WESTERN immunoblotting; RETINA
- Publication
Journal of Ophthalmology, 2024, Vol 2024, p1
- ISSN
2090-004X
- Publication type
Article
- DOI
10.1155/2024/3654690