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- Title
Pharmacological inhibition of Lipid Droplets biogenesis and its impact in the replication of Respiratory Syncytial Virus.
- Authors
Herrera Moro-Huitron, L. A.; Ulloa-Aguilar, J. M.; Vargas-Pavía, T. A.; Velázquez-Cervantes, M. A.; Benítez-Zeferino, R. Y.; De Jesús-González, L. A.; Helguera-Repetto, A. C.; García-Cordero, J.; Cedillo-Barrón, L.; Castillo Martínez, Macario; Arévalo-Romero, H.; Cerna Cortés, J. F.; León-Juárez, M.
- Abstract
The viruses can recruit cellular factors for their infection cycle to be successful. In this sense, lipid droplets (LD), which are cellular structures that store lipids and can function as an energy reservoir, play an essential role during viral infections. These structures may be involved in viral particles’ entry, assembly, replication, and exit during infections. We characterized the effects of biogenesis and pharmacological blockade of LD and its impact on replicating the Respiratory Syncytial Virus (RSV) in a pulmonary epithelial cell line (A549). As a result, we observed that in RSV-infected cells, there is a decrease in LD and a change in the spatial distribution pattern. The LD biogenesis caused an increase in the number of RSV-infected cells. The pharmacological inhibition of LD caused a reduction in the expression of viral M2-1 protein and significant downregulation of the RSV viral F protein gene. Finally, we found a decrease in PFU in cells treated with LD inhibitor compared to control cells. The results show that RSV infection in A549 cells can promote a reduction of lipid droplets, and pharmacological inhibition of lipid droplets has a negative effect on viral replication and the production of infective viral particles.
- Publication
Veterinaria México OA, 2024, Vol 11, p7
- ISSN
2448-6760
- Publication type
Article
- DOI
10.22201/fmvz.24486760e.2024.1305