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- Title
Single-cell transcriptomics reveals tumor-infiltrating B cell function after neoadjuvant pembrolizumab and chemotherapy in non-small cell lung cancer.
- Authors
Hou, Lingjie; Zhang, Siyuan; Yu, Wenwen; Yang, Xuena; Shen, Meng; Hao, Xishan; Ren, Xiubao; Sun, Qian
- Abstract
Non-small cell lung cancer (NSCLC) is the most pervasive lung cancer subtype. Recent studies have shown that immune checkpoint inhibitors achieved favorable clinical benefits in resectable NSCLC; however, the associated mechanism remains unclear. The role of T cells in antitumor immunity has received considerable attention, while the antitumor effects of tumor-infiltrating B cells (TIBs) in NSCLC remain poorly understood. Here, we conducted a single-cell RNA sequencing analysis of immune cells isolated from 12 patients with stage IIIA NSCLC to investigate B cell subtypes and their functions following neoadjuvant chemoimmunotherapy. We confirmed the simultaneous existence of the 4 B cell subtypes. Among them, memory B cells were found to be associated with a positive therapeutic effect to neoadjuvant chemoimmunotherapy. Furthermore, we found that G protein–coupled receptor 183 was most prevalent in memory B cells and associated with a positive therapeutic response. Multiplex immunofluorescence and flow cytometry experiments in an additional cohort of 22 treatment-naïve and 30 stage IIIA/IIIB NSCLC patients treated with neoadjuvant chemoimmunotherapy verified these findings. Overall, our analysis revealed the functions of TIBs and their potential effect on clinical treatment in NSCLC.
- Subjects
NON-small-cell lung carcinoma; IMMUNOLOGIC memory; B cells; IMMUNE checkpoint inhibitors; TREATMENT effectiveness
- Publication
Journal of Leukocyte Biology, 2024, Vol 116, Issue 3, p555
- ISSN
0741-5400
- Publication type
Article
- DOI
10.1093/jleuko/qiad138