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- Title
Isolation of 2,2′-azoxybisbenzyl alcohol from Agaricus subrutilescens and its inhibitory activity against bacterial biofilm formation.
- Authors
Padua, Jewel C De; Tanaka, Tomoya; Ueno, Kotomi; Cruz, Thomas Edison E dela; Ishihara, Atsushi
- Abstract
Virulence pathways in pathogenic bacteria are regulated by quorum sensing mechanisms, particularly biofilm formation through autoinducer (AI) production and sensing. In this study, the culture filtrate extracted from an edible mushroom, Agaricus subrutilescens , was fractionated to isolate a compound that inhibits biofilm formation. Four gram-negative bacteria (Klebsiella pneumoniae, Escherichia coli, Proteus mirabilis , and Enterobacter cloacae) and two gram-positive bacteria (Enterococcus faecalis and Staphylococcus aureus) were used for the bioassay. The bioassay-guided chromatographic separations of the culture filtrate extract resulted in the isolation of the compound. Further, spectroscopic analyses revealed the identity of the compound as 2,2′-azoxybisbenzyl alcohol (ABA). The minimum inhibitory and sub-inhibitory concentrations of the compound were also determined. Azoxybisbenzyl alcohol was significantly effective in inhibiting biofilm formation in all tested bacteria, with half-maximal inhibitory concentrations of 3–11 µg/mL. Additionally, the bioactivity of ABA was confirmed through the bioassays for the inhibition of exopolysaccharide matrixes and AI activities.
- Subjects
GRAM-positive bacteria; PATHOGENIC bacteria; GRAM-negative bacteria; QUORUM sensing; ENTEROBACTER cloacae; EDIBLE mushrooms
- Publication
Bioscience, Biotechnology & Biochemistry, 2024, Vol 88, Issue 9, p983
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1093/bbb/zbae089