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- Title
Expression analysis of gibberellin-regulated protein in peach by reverse transcription-quantitative PCR.
- Authors
Natsuki Kawai; Yuki Hirakawa; Shinya Matsumoto; Akihiro Itai; Kayoko Matsunaga; Hiroshi Narita; Keiko Momma
- Abstract
Gibberellin-regulated protein (GRP) is a fruit severe allergen. The amounts of GRP expression normalized against actin in peach were determined by reverse transcription-quantitative PCR (RT-qPCR). The results were consistent with those determined by enzyme-linked immunosorbent assay (ELISA). The GRP expression was more evident in flesh than peel and increased rapidly in the maturing period. This approach is applicable to estimate the amount of GRP in other plants.
- Subjects
REVERSE transcriptase polymerase chain reaction; PROTEIN analysis; PEACH; ENZYME-linked immunosorbent assay
- Publication
Bioscience, Biotechnology & Biochemistry, 2022, Vol 86, Issue 10, p1459
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1093/bbb/zbac123