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- Title
The eIF4E-binding protein Eap1 has similar but independent roles in cell growth and gene expression with the cytoplasmic deadenylase Ccr4.
- Authors
Higuchi, Yudai; Fujii, Shiori; Valderrama, Arvin Lapiz; Irie, Kaoru; Suda, Yasuyuki; Mizuno, Tomoaki; Irie, Kenji
- Abstract
eIF4E-binding proteins (4E-BPs) are translational repressors that compete with eIF4G for binding to eIF4E. Here we investigated the roles of yeast 4E-BPs, Eap1, and Caf20 in cell wall integrity pathway and gene expression. We found that eap1∆ mutation, but not caf20∆ mutation, showed synthetic growth defect with mutation in ROM2 gene encoding Rho1 GEF. The eap1∆ mutation also showed synthetic lethality with mutation in CCR4 gene encoding cytoplasmic deadenylase. Ccr4 functions in the degradation of LRG1 mRNA encoding Rho1 GAP. Eap1-Y109A L114A, which could not bind to eIF4E, did not suppress the synthetic lethality of eap1∆ ccr4∆ mutant, suggesting that 4E-binding of Eap1 is important for its function. We also found that eap1∆ mutant showed the derepression of stress response gene HSP12. 4E-binding of Eap1 was also required for the repression of HSP12 expression. Our results indicate that Eap1 has similar but independent roles in cell growth and gene expression with Ccr4.
- Subjects
GENE expression; CHEMOKINE receptors; CELL growth; PROTEINS; GENETIC mutation
- Publication
Bioscience, Biotechnology & Biochemistry, 2021, Vol 85, Issue 6, p1452
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1093/bbb/zbab056