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- Title
Inhibition of human neutrophil elastase by labdane diterpenes from the fruiting bodies of <italic>Ramaria formosa</italic>.
- Authors
Lee, Ik-Soo; Kim, Kwan-Chul; Yoo, Ick-Dong; Ha, Byung-Jo
- Abstract
Two new labdane diterpenes (<bold>1</bold> and <bold>2</bold>) were isolated from the fruiting bodies of <italic>Ramaria formosa</italic>. The structures of these compounds were established by extensive spectroscopic studies and chemical evidence. The inhibitory activity of compounds <bold>1</bold> and <bold>2</bold> against human neutrophil elastase (HNE) was evaluated <italic>in vitro</italic>. Compounds <bold>1</bold> and <bold>2</bold> inhibited HNE activity moderately. The IC50 values for compounds <bold>1</bold> and <bold>2</bold> were 36.4 ± 1.2 and 40.8 ± 1.5 μM, respectively; the IC50 value for the positive control, EGCG, was 12.5 ± 0.8 μM. In addition, the mechanism by which <bold>2</bold> inhibited HNE was a mixed-type noncompetitive inhibition, with a <italic>K</italic>i of 41.5 ± 1.8 μM.
- Subjects
DITERPENES; LEUCOCYTE elastase; GOMPHACEAE
- Publication
Bioscience, Biotechnology & Biochemistry, 2015, Vol 79, Issue 12, p1921
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2015.1065168