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- Title
Cellular Synthesis of Protein Catenanes.
- Authors
Wang, Xiao ‐ Wei; Zhang, Wen ‐ Bin
- Abstract
Direct cellular production of topologically complex proteins is of great interest both in supramolecular chemistry and protein engineering. We describe the first cellular synthesis of protein catenanes through the use of the p53 dimerization domain to guide the intertwining of two protein chains and SpyTag-SpyCatcher chemistry for efficient cyclization. The catenane topology was unambiguously proven by SDS-PAGE, SEC, and partial digestion experiments and was shown to confer enhanced stability toward trypsin digestion relative to monomeric control mutants. The assembly-reaction synergy enabled by protein folding and genetically encoded protein chemistry offers a convenient yet powerful approach for creating mechanically interlocked, complex protein topologies in vivo.
- Subjects
CATENANES; PROTEIN research; SUPRAMOLECULAR chemistry; RING formation (Chemistry); CHEMICAL synthesis
- Publication
Angewandte Chemie, 2016, Vol 128, Issue 10, p3503
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201511640