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- Title
Molecular variation at BvBTC1 is associated with bolting tolerance in Japanese sugar beet.
- Authors
Kuroda, Yosuke; Takahashi, Hiroyuki; Okazaki, Kazuyuki; Taguchi, Kazunori
- Abstract
Bolting tolerance is an important breeding trait in sugar beets (Beta vulgaris). To understand factors controlling bolting tolerance in Japanese sugar beets, we focused on the molecular variation of the BvBTC1 gene, a master gene linked to life cycle (annual vs. biennial). Although the biennial haplotype "a" is recognized in beets worldwide, our genomic sequencing detected two additional haplotypes, an annual haplotype "g" and a novel haplotype we named "o", which is similar to the haplotype found in annual beet. We developed cleaved amplified polymorphic sequence markers to discriminate haplotypes 'a', 'g' and 'o'. Their correlation with bolting tendency was investigated by analyzing bolting-induced beets. In segregated genotypes (hybrid or strains), we found that bolting plants tended to possess a lower proportion of the biennial "aa" genotype than did non-bolting plants. We also observed that bolting tolerance varied among inbred breeding strains (strains whose genotypes were mostly fixed). Genotype frequencies of "aa" were lower in strains with weaker bolting tolerances (88.9% frequency for strong tolerance, 42.2% for medium tolerance, and 19.3% for weak tolerance). Therefore, molecular variation in the BvBTC1 gene can be a target for improving bolting tolerance in Japanese sugar beets.
- Subjects
SUGAR beets; GENOTYPES; BOLTING (Botany); HAPLOTYPES; VERNALIZATION; GENES; NUCLEOTIDE sequencing
- Publication
Euphytica, 2019, Vol 215, Issue 3, p1
- ISSN
0014-2336
- Publication type
Article
- DOI
10.1007/s10681-019-2366-9