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- Title
Chromogenic analogues of penicillin dihydroF and penicillin K for the continuous spectrophotometric determination of aliphatic penicillin acylase activity.
- Authors
Arroyo, Miguel; Torres-Guzmán, Raquel; Torres-Bacete, Jesús; de la Mata, Isabel; Pilar Castillón, María; Acebal, Carmen
- Abstract
The synthesis of 2-nitro-5-[(hexanoyl)-amino]-benzoic acid and 2-nitro-5-[(octanoyl)-amino]-benzoic acid as chromogenic substrates for the determination of aliphatic penicillin acylase activity is described. During enzymatic hydrolysis, the released chromophore, 2-nitro-5-amino-benzoic acid, was detected at 405 nm. Penicillin acylase from Streptomyces lavendulae had an appreciable activity towards these substrates, which can then be used to detect penicillin acylases able to cleave hexanoyl and octanoyl residues off synthetic amides as well as penicillin dihydroF and penicillin K, their natural analogues.
- Subjects
PENICILLIN; ANTIBACTERIAL agents; ANTI-infective agents; BETA lactam antibiotics; HYDROLYSIS; AMIDES
- Publication
Biotechnology Letters, 2002, Vol 24, Issue 13, p1045
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1016096111453