We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Synthesis of recombinant human interleukin-2 via controlled feed of lactose-complex media in fed-batch cultures of Escherichia coli BL21(DE3)[pT7-G3IL2].
- Authors
Saraswat, Vibhor; Lee, Jeewon; Kim, Dae Young; Park, Young-Hoon
- Abstract
Using lactose as an inducer, recombinant human interleukin-2 (rhIL-2) was synthesized with an N-terminus fusion partner, G3 (three tandem-arranged glucagon peptides) in fed-batch cultures at high cell concentration (60–90 g l-1) of Escherichia coli BL21(DE3) [pT7-G3IL2]. With batch additions of lactose (4 × 13.5 g), the fusion rhIL-2 was synthesized up to 9.3 g l-1. However, if all the lactose (54 g) was added at once to the culture, synthesized fusion rhIL-2 decreased to 5.4 g l-1 with a decreased cell growth rate. A statistical optimization of the production medium containing glucose, yeast extract, and lactose led to fusion rhIL-2 being produced at > 9 g l-1.
- Subjects
LACTOSE; INTERLEUKIN-2; ESCHERICHIA coli; CELL culture; PEPTIDES; CELL growth
- Publication
Biotechnology Letters, 2000, Vol 22, Issue 4, p261
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1005638602146