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- Title
Characterisation of several heterogeneous species of defective RNAs derived from RNA 3 of cucumber mosaic virus.
- Authors
López, C.; Aramburu, J.; Galipienso, L.; Nuez, F.
- Abstract
Preparations of double-stranded RNAs (dsRNAs) extracted from Nicotiana tabacum cv Xanthi plants infected with a subgroup IB isolate of Cucumber mosaic virus (CMV) were found to contain a heterogeneous population of defective RNAs (D-RNAs) derived from RNA 3. Characterised D-RNAs ranged in size from 1.5 to 1.9 kb and were derived either by a single in-frame deletion within the 3a or 3b genes or by means of double in-frame deletions within both genes. Also, northern blot hybridisation showed two other types of RNA derived from RNA 3: (a) RNA species of ca. 0.7 kb containing the 3′-terminus but lacking the 5′-terminus, which could be 3′-coterminal subgenomic of D-RNAs derived from the 3b gene and (b) RNA species of unknown origin of ca. 0.8 kb containing the 5′-terminus but lacking the 3′-terminus.
- Subjects
PLANT viruses; CUCUMBER mosaic virus; TOBACCO diseases &; pests; MOLECULAR biology; MOLECULAR genetics; NUCLEIC acids
- Publication
Archives of Virology, 2007, Vol 152, Issue 3, p621
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-006-0875-z