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- Title
厚褶小奥德蘑栽培及其抗氧化活性.
- Authors
史钏; 胡惠萍; 莫伟鹏; 刘远超; 谢意珍
- Abstract
A wild strain of Oudemansiella sp.was collected from Dinghushan national nature reserve in Guangdong Province and identified as O. crassifolia based on morphological observations and rDNA-ITS sequence analysis. Then this strain was studied for artificial cultivation and the antioxidant activity of its water extract, ethanol extract and mycelial fermentation broth, respectively. The results showed that this strain of O. crassifolia was successfully cultivated on a substrate comprising 58%wood chips, 30% cottonseed hull, 10% wheat bran, and 2% calcium carbonate with 65% moisture and natural pH. The cultivation was first carried out in shade at(25±1)°C for 20 d to allow full growth of mycelia in cultivation bags. Then the bags were incubated under the same condition for another 15-16 d prior to transference to the mushroom chamber for further development of mushroom buds under 25-28 °C, relative humidity 85%-90%, and 300-500 lx light for 10 h each day. Upon development of mushroom buds, the mushroom chamber was ventilated 3-4 times a day for fruit body maturation. The mature fruit bodies were collected for 3-4 flushes and the overall biological efficiency was 79.15%. Among all tested samples, the reducing power and hydroxyl radical scavenging ability of water extract of O. crassifolia mycelia were significantly higher than that of mycelium ethanol extract and fermentation broth. Compared with vitamin C, water extract of O. crassifolia mycelia showed lower reducing power. On the other hand, its hydroxyl radical scavenging ability was 1.81 times of vitamin C.
- Subjects
GUANGDONG Sheng (China); HYDROXYL group; BUD development; VITAMIN C; NATURE reserves; NATIONAL parks &; reserves; RICE hulls; WHEAT bran; CALCIUM carbonate
- Publication
Acta Edulis Fungi, 2019, Vol 26, Issue 4, p78
- ISSN
1005-9873
- Publication type
Article
- DOI
10.16488/j.cnki.1005-9873.2019.04.011