We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Impact of Aging and HIV Infection on the Function of the C-Type Lectin Receptor MINCLE in Monocytes.
- Authors
Zapata, Heidi J; Siconolfi, Barbara; Wilson, Jean; Mohanty, Subhasis; Shaw, Albert C; Ness, Peter H Van; Allore, Heather G; Tsang, Sui; Avey, Stefan; Barakat, Lydia; Van Ness, Peter H
- Abstract
Both aging and HIV infection are associated with an enhanced pro-inflammatory environment that contributes to impaired immune responses and is mediated in part by innate immune pattern-recognition receptors. MINCLE is a C-type lectin receptor that recognizes trehalose-6,6'-dimycolate or "cord factor," the most abundant glycolipid in Mycobacterium tuberculosis. Here, we evaluated MINCLE function in monocytes in a cohort of HIV-infected and uninfected young (21-35 years) and older adults (≥60 years) via stimulation of peripheral blood mononuclear cells with trehalose-6,6-dibehenate, a synthetic analog of trehalose-6,6'-dimycolate and measurement of cytokine production (interleukin [IL]-10, IL-12, IL-6, tumor necrosis factor-α) by multicolor flow cytometry. Our studies show an age- and HIV-associated increase in cytokine multifunctionality of monocytes both at the population and single cell level that was dominated by IL-12, IL-10, and IL-6. These findings provide insight into the host response to M. tuberculosis and possible sources for the pro-inflammatory environment seen in aging and HIV infection.
- Subjects
HIV infections; MONOCYTES; CELL populations; OLDER people; MYCOBACTERIUM tuberculosis
- Publication
Journals of Gerontology Series A: Biological Sciences & Medical Sciences, 2019, Vol 74, Issue 6, p794
- ISSN
1079-5006
- Publication type
journal article
- DOI
10.1093/gerona/gly209