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- Title
Serotonin in Platelets: Comparative Analyses Using New Enzyme Immunoassay and HPLC Test Kits and the Traditional Fluorimetric Procedure.
- Authors
Kluge, H.; Bolle, M.; Reuter, R.; Werner, S.; Zahlten, W.; Prudlo, J.
- Abstract
Recently new test kits for an enzyme im-munoassay and for an HPLC procedure to determine serotonin in different fluids and tissues were introduced to the market. Its applicability for the serotonin determination in isolated platelets was not separately described, thus above all stimulating us to elaborate conditions for the extraction of platelet pellets for both methods. Our extraction method for the immunoassay based on hypotonic lysis in distilled water coupled with freezing/thawing at -20 °C was already worked into the corresponding instruction manual. Including the traditional fluorimetric test, the serotonin concentrations of 50 platelet samples were simultaneously determined. An excellent correlation could be observed between HPLC and immunoassay values (r = 0.9919), between HPLC and fluorimetric values (r = 0.9857), and between immunoassay and fluorimetric values (r = 0.9856). Related to the HPLC values (= 100%), neither those of the immunoassay nor those of the fluorimetric procedure differed significantly (mean of the differences ± S.EM. = 0.3 ± 0.9% and -0.8 ± 0.8%, respectively). The variation coefficients from intra- and inter-assay series amounted to about 8% for the immunoassay and 5% for the HPLC and the fluorimetric method. Based on our data, the three methods compared are qualified for a reliable determination of serotonin in platelets.
- Publication
Journal of Laboratory Medicine / Laboratoriums Medizin, 1999, Vol 23, Issue 6, p360
- ISSN
0342-3026
- Publication type
Article