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- Title
顺铂短期诱导人腺样囊性癌细胞 NACC 产生耐药性的研究.
- Authors
陈虎; 农晓琳; 陈宇麟; 李佳荃
- Abstract
Objective: To study the effect of transient exposure to cisplatin on drug resistance of novel human palatal salivary gland cell line of adenoid cystic carcinoma (NACC) and the mechanism of drug resistance. Methods: The DDP-resistant cell line NACC/DDP3 was established by constant-dose cisplatin intermittent selection from its parental cell NACC. The resistance index was determined by MTT, the mRNA expression of Survivin and ERCC 1 was analyzed by real-time quantitive PCR. Tumor cells were injected subcutaneously in the right axillary of nude mice, the xenografling mice were randomly divided into the control group and 2 mg. kg~ DDP treated group after volume of solid tumors reached 0.5 cm3, tumor growth inhibition rates of 2 mg. kg-1 DDP on xenografting mice were compared, then the tumor samples were stained by hematoxylin-eosin (HE) after the tumors were excised. Results: Compared with NACC cells, the NACC/DDP3 cells showed significantly higher drug-resistance, the resistance index was 1.44, the mRNA expression of Survivin and ERCC 1 in NACC/DDP3 was also augmented, the expression level of Survivin and ERCC 1 in NACC/DDP3 was 2.02 (P<0. 01) and 1.59 (P<0.05) fold of NACC, respectively. The xenografting mice of NACC group treated with DDP at 2 mg .kg-1 showed significantly higher tumor growth inhibition[(31.64+ 1.15) % at day 30] than NACC/DDP3 group[(16.66+ 0.76) % at day 30](P<0.01). Two group samples were histologically similar to the phenotype of the solid type of adenoid cystic carcinoma (ACC). Conclusions: The phenotype of drug-resistance of NACC can be induced by transient exposure to cisplatin, the drug-resistance of NACC/DDP3 might related to the over expression of mRNA of Survivin and ERCC1. The subcutaneous xenograft of NACC/DDP3 can provide an ideal model for reversing cisplatin resistance of ACC in vivo for the NACC/DDP3 maintained the drug-resistance phenotype when inoculated into nude mice.
- Publication
Progress in Modern Biomedicine, 2016, Vol 16, Issue 14, p2641
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2016.14.010