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- Title
Genetic analysis of the physiological responses to low boron stress in Arabidopsis thaliana.
- Authors
CHANGYING ZENG; YANLAI HAN; LEI SHI; LISHUN PENG; YUNHUA WANG; FANGSEN XU; JINLING MENG
- Abstract
Boron (B) is an essential micronutrient for higher plants. There is wide genetic variation in the response to B deficiency among plant species and cultivars. The objective of this study was to identify quantitative trait loci (QTL) that control B efficiency in natural Arabidopsis accessions. The B efficiency coefficient (BEC) and seed yield under low B conditions (SYLB) were investigated by solution culture in two separate experiments in an Arabidopsis recombinant inbred line (RIL) population. Both of the traits studied exhibited high transgressive variation in the RIL population, and, in total, five and three QTL were identified for BEC and SYLB, respectively. Three of the five QTL, including the QTL, AtBE1-2, that has a large effect on the BEC, were found at the interval of the corresponding QTL for SYLB in both experiments. The close genetic relationship between BEC and SYLB was further confirmed by conditional QTL mapping in the RIL population and unconditional QTL mapping in an AtBE1-2-segregated F2 population. Epistatic interactions for the tested traits were analysed, and were found to be widespread in the detected QTL of Arabidopsis in the RIL population. Comparison of the QTL interval for B efficiency with reported B-related genes showed that 10 B-related genes, together with one BOR1 homolog ( BOR5, At1g74810) were located in the QTL region of AtBE1-2. These results suggest that natural variation in B efficiency in Arabidopsis has a complex molecular basis. They also provide a basis for fine mapping and cloning of the B-efficiency genes, with the ultimate aim of discovering the physiological mechanism of action of the genes.
- Subjects
BORON; ARABIDOPSIS thaliana; MICRONUTRIENTS; TRACE elements; PLANT ecology; OILSEED plants; BOTANY; PLANT physiology; MOLECULAR biology
- Publication
Plant, Cell & Environment, 2008, Vol 31, Issue 1, p112
- ISSN
0140-7791
- Publication type
Article
- DOI
10.1111/j.1365-3040.2007.01745.x