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- Title
Reactive oxygen species stimulate homologous recombination in plants.
- Authors
Kovalchuk, I.; Filkowski, J.; Smith, K.; Kovalchuk, O.
- Abstract
ABSTRACT Coping with the continuous production of free radicals is a daily routine of the cell. Despite their toxicity, the reactive oxygen species (ROS) are involved in dual physiological action – signal transduction and immune response. We analysed the influence of oxidative stress-generating compounds, rose Bengal (RB), paraquat (PQ) and amino-triazole (ATZ) on the genome stability of Arabidopsis using transgenic recombination-monitoring plants. Homologous recombination frequencies in plants were increased upon the treatment with RB and PQ but not ATZ. Application of the N -acetyl-L-cysteine (NAC), radicals scavenging compound, decreased the DNA damage caused by RB. Interestingly, the incubation of plants with very low concentration of RB (less than 0.2 µ M) led to the subsequent increase in plant tolerance to methyl methane sulfonate (MMS): stronger plants with a lower increase of homologous recombination frequency. In contrast, the incubation of plants with 0.5 µ M of RB resulted in the potentiation of the MMS effect: the weaker plants with higher frequency of recombination. The data of the present study suggest the existence of a dual concentration-dependent role of ROS in plants.
- Subjects
REACTIVE oxygen species; PLANT genomes; ARABIDOPSIS; ROSE bengal; PARAQUAT
- Publication
Plant, Cell & Environment, 2003, Vol 26, Issue 9, p1531
- ISSN
0140-7791
- Publication type
Article
- DOI
10.1046/j.1365-3040.2003.01076.x