We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Siraitia grosvenorii Extract Attenuates Airway Inflammation in a Murine Model of Chronic Obstructive Pulmonary Disease Induced by Cigarette Smoke and Lipopolysaccharide.
- Authors
Kim, Mi-Sun; Kim, Dong-Seon; Yuk, Heung Joo; Kim, Seung-Hyung; Yang, Won-Kyung; Park, Geum Duck; Kim, Kyung Seok; Ham, Woo Jung; Sung, Yoon-Young
- Abstract
We studied the activities of Siraitia grosvenorii extracts (SGE) on airway inflammation in a mouse model of chronic obstructive pulmonary disease (COPD) stimulated by cigarette smoke extract (CSE) and lipopolysaccharide (LPS), as well as in LPS-treated human bronchial epithelial cell line (BEAS-2B). SGE improved the viability of LPS-incubated BEAS-2B cells and inhibited the expression and production of inflammatory cytokines. SGE also attenuated the mitogen-activated protein kinase (MAPK)-nuclear factor-kappa B (NF-κB) signaling activated by LPS stimulation in BEAS-2B cells. In mice stimulated by CSE and LPS, we observed the infiltration of immune cells into the airway after COPD induction. SGE reduced the number of activated T cells, B cells, and neutrophils in bronchoalveolar fluid (BALF), lung tissue, mesenteric lymph node, and peripheral blood mononuclear cells, as well as inhibited infiltration into organs and mucus production. The secretion of cytokines in BALF and the expression level of pro-inflammatory cytokines, mucin 5AC, Transient receptor potential vanilloid 1, and Transient receptor potential ankyrin 1 in lung tissue were alleviated by SGE. In addition, to investigate the activity of SGE on expectoration, we evaluated phenol red secretions in the trachea of mice. SGE administration showed the effect of improving expectoration through an increase in phenol red secretion. Consequently, SGE attenuates the airway inflammatory response in CSE/LPS-stimulated COPD. These findings indicate that SGE may be a potential herbal candidate for the therapy of COPD.
- Subjects
LIPOPOLYSACCHARIDES; BIOLOGICAL models; CYTOKINES; HERBAL medicine; INFLAMMATION; ANIMAL experimentation; NF-kappa B; CELLULAR signal transduction; OBSTRUCTIVE lung diseases; BRONCHI; RESEARCH funding; PLANT extracts; SMOKING; EPITHELIAL cells; CELL lines; MITOGEN-activated protein kinases; MICE
- Publication
Nutrients, 2023, Vol 15, Issue 2, p468
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu15020468