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- Title
Influence of Fermented Red Clover Extract on Skeletal Muscle in Early Postmenopausal Women: A Double-Blinded Cross-Over Study.
- Authors
Oxfeldt, Mikkel; Dalgaard, Line Barner; Risikesan, Jeyanthini; Johansen, Frank Ted; Hansen, Mette
- Abstract
Objective: To investigate effects of supplementation with a fermented red clover (RC) extract on signaling proteins related to muscle protein synthesis and breakdown at rest and in response to a resistance exercise bout. Methods: Ten postmenopausal women completed a double-blinded cross-over trial with two different intervention periods performed in random order: (A) RC extract twice daily for 14 days, and (B) placebo drink twice daily for 14 days. The intervention periods were separated by a two-week washout period. After each intervention period a muscle tissue sample was obtained before and three hours after a one-legged resistance exercise bout. Muscle strength was assessed before and after each intervention period. Results: Protein expression of FOXO1 and FOXO3a, two key transcription factors involved in protein degradation, were significantly lower and HSP27, a protein involved in cell protection and prevention of protein aggregation was significantly higher following RC extract compared to placebo. No significant treatment × time interaction was observed for muscle protein expression in response to exercise. However, p-mTOR, p-p70S6k and HSP90 protein content were significantly increased in response to exercise in both groups. Conclusion: This study demonstrates that RC extract supplementation downregulates molecular markers of muscle protein degradation compared to placebo in postmenopausal women.
- Subjects
CROSSOVER trials; DIETARY supplements; GENE expression; MUSCLE strength; PLACEBOS; RED clover; STATISTICAL sampling; T-test (Statistics); TRANSCRIPTION factors; WESTERN immunoblotting; WOMEN'S health; PLANT extracts; ISOFLAVONES; PHYTOESTROGENS; RANDOMIZED controlled trials; PRE-tests &; post-tests; REPEATED measures design; BLIND experiment; POSTMENOPAUSE; DATA analysis software; SKELETAL muscle; DESCRIPTIVE statistics
- Publication
Nutrients, 2020, Vol 12, Issue 11, p3587
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu12113587