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- Title
GFP-tagged multimetal-tolerant bacteria and their detection in the rhizosphere of white mustard.
- Authors
Piotrowska-Seget, Zofia; Beściak, Grażyna; Bernaś, Tytus; Kozdrój, Jacek
- Abstract
The introduction of rhizobacteria that tolerate heavy metals is a promising approach to support plants involved in phytoextraction and phytostabilisation. In this study, soil of a metal-mine wasteland was analyzed for the presence of metal-tolerant bacterial isolates, and the tolerance patterns of the isolated strains for a number of heavy metals and antibiotics were compared. Several of the multimetal-tolerant strains were tagged with a broad host range reporter plasmid (i.e. pPROBE-NT) bearing a green fluorescent protein marker gene ( gfp). Overall, the metal-tolerant isolates were predominately Gram-negative bacteria. Most of the strains showed a tolerance to five metals (Zn, Cu, Ni, Pb and Cd), but with differing tolerance patterns. From among the successfully tagged isolates, we used the transconjugant Pseudomonas putida G25 (pPROBE-NT) to inoculate white mustard seedlings. Despite a significant decrease in transconjugant abundance in the rhizosphere, the gfp-tagged cells survived on the root surfaces at a level previously reported for root colonisers.
- Publication
Annals of Microbiology, 2012, Vol 62, Issue 2, p559
- ISSN
1590-4261
- Publication type
Article
- DOI
10.1007/s13213-011-0292-8