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- Title
Diagnosis of Chlamydia trachomatis infections by direct immunofluorescence staining of genital secretions. A multicenter trial.
- Authors
Stamm, Walter E.; Harrison, H. Robert; Alexander, E. Russell; Cles, Linda D.; Spence, Michael R.; Quinn, Thomas C.; Stamm, W E; Harrison, H R; Alexander, E R; Cles, L D; Spence, M R; Quinn, T C
- Abstract
Because few clinicians have access to laboratories offering cell culture confirmation of suspected Chlamydia trachomatis genital infections, we evaluated a diagnostic method in which fluorescein-conjugated monoclonal antibodies were used to directly identify C. trachomatis elementary bodies in slides made from genital secretions. Compared with culture results, the direct smear had a sensitivity of 92% and a specificity of 96% in 576 men, most of whom had symptoms and signs of urethritis. Among 595 women attending the same clinics, sensitivity of the direct smear for cervical infection was 89% and specificity was 99%. In 225 pregnant women screened in a prenatal or abortion clinic, the sensitivity and specificity of the test were 86% and 99% respectively. Direct detection of elementary bodies in genital smears offers an alternative diagnostic approach for C. trachomatis infections.
- Subjects
FLUORESCEIN; MONOCLONAL antibodies; CHLAMYDIA infection diagnosis; BACTERIOLOGY technique; CELLS; CERVIX uteri; CHLAMYDIA infections; CHLAMYDIA trachomatis; COMMUNICABLE diseases; COMPARATIVE studies; FLUORESCENT antibody technique; RESEARCH methodology; MEDICAL cooperation; PELVIC inflammatory disease; PREGNANCY complications; RESEARCH; SEXUALLY transmitted diseases; URETHRA; URETHRITIS; EVALUATION research
- Publication
Annals of Internal Medicine, 1984, Vol 101, Issue 5, p638
- ISSN
0003-4819
- Publication type
journal article
- DOI
10.7326/0003-4819-101-5-638