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- Title
UV-A light regulation of arylalkylamine N-acetyltransferase activity in the chick pineal gland: role of cAMP and proteasomal proteolysis.
- Authors
Rosiak, Jolanta; Michael Iuvone, P.; Zawilska, Jolanta B.
- Abstract
Acute exposure of dark-adapted, cultured chick pineal glands to UV-A light significantly decreased the tissue cAMP concentration and the activity of arylalkylamine N-acetyltransferase (AANAT), the penultimate and key regulatory enzyme in the melatonin biosynthetic pathway. The magnitude of these changes was dependent on the duration of UV-A exposure. The UV-A light-evoked decline in pineal AANAT activity was blocked by cAMP protagonists (forskolin and dibutyryl-cAMP) and by inhibitors of the proteasomal degradation pathway (MG-132, proteasome inhibitor I, and lactacystin). These results indicate that the chick pineal gland is directly sensitive to UV-A light. By analogy to white light, the suppressive action of UV-A radiation on AANAT activity in the chick pineal gland involves changes in the tissue cAMP level and enhanced proteasomal proteolysis.
- Subjects
PINEAL gland; CYCLIC adenylic acid; MELATONIN; ULTRAVIOLET radiation; PROTEOLYSIS; ACETYLTRANSFERASES
- Publication
Journal of Pineal Research, 2005, Vol 39, Issue 4, p419
- ISSN
0742-3098
- Publication type
Article
- DOI
10.1111/j.1600-079X.2005.00267.x