We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Effect of avocado/soybean unsaponifiables on ligature-induced bone loss and bone repair after ligature removal in rats.
- Authors
Oliveira, G. J. P. L.; Paula, L. G. F.; Souza, J. A. C.; Spin‐Neto, R.; Stavropoulos, A.; Marcantonio, R. A. C.
- Abstract
Background and Objective The aim of this study was to evaluate the effects of administration of avocado/soybean unsaponifiable ( ASU), a drug that is commonly used in the treatment of rheumatoid arthritis, on ligature-induced bone loss and bone repair after ligature removal in rats. Material and Methods Eighty-four rats were randomly assigned to four groups of equal size and received a daily gavage of either sterile saline [control ( CTR)] or ASU (0.6 mg/kg), starting 7 d before ( ASU/−7), on the day of ( ASU/0) or 7 d after ( ASU/+7) periodontitis induction. Periodontitis was induced by placing silk ligatures into the gingival sulcus of the second maxillary molars for 7 d; after 7 d, the ligatures were removed. Seven rats from each group were sacrificed, 7, 15 or 30 d after ligature removal. Bone resorption was evaluated by histomorphometry and micro-computed tomography (micro- CT). Immunohistochemistry was used to evaluate the expression of TRAP, RANKL and alkaline phosphatase (ALP), and quantitative PCR ( qPCR) was used to evaluate the levels of interleukin-1beta ( Il1β), tumor necrosis factor alpha (Tnfα), interleukin-6 (Il-6), Rankl and Alp. Statistical analysis was performed using the Shapiro-Wilk test, ANOVA and Tukey's test for normal data, and using the Kruskall-Wallis and Dunnet's tests for non-normal data ( p < 0.05). Results Histomorphometry and micro- CT analysis showed greater bone resorption in the CTR group than in the ASU/0 (15 d) and ASU/+7 (7 and 15 d) groups. The CTR group also presented with a higher expression of TRAP (15 and 30 d) and RANKL (7 and 15 d) compared with ASU/0 and ASU/+7 groups. Similarly, qPCR analysis showed higher levels of Rankl and Il1β mRNAs, and lower levels of Alp mRNA, in the CTR group compared with all other groups (for all periods). Conclusion ASU exhibited a positive effect on bone repair following ligature-induced periodontitis in rats.
- Subjects
ANIMAL experimentation; AVOCADO; STATISTICAL correlation; GENE expression; HISTOLOGY; IMMUNOHISTOCHEMISTRY; LIGATURE (Surgery); RATS; SOYBEAN; STATISTICS; T-test (Statistics); WOUND healing; DATA analysis; MANN Whitney U Test; KRUSKAL-Wallis Test
- Publication
Journal of Periodontal Research, 2016, Vol 51, Issue 3, p332
- ISSN
0022-3484
- Publication type
Article
- DOI
10.1111/jre.12312