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- Title
Nitric oxide inhibits androgen receptor-mediated collagen production in human gingival fibroblasts.
- Authors
Lin, S‐J.; Lu, H‐K.; Lee, H‐W.; Chen, Y‐C.; Li, C‐L.; Wang, L‐F.
- Abstract
Lin S-J, Lu H-K, Lee H-W, Chen Y-C, Li C-L, Wang L-F. Nitric oxide inhibits androgen receptor-mediated collagen production in human gingival fibroblasts. J Periodont Res 2012; 47: 701-710. © 2012 John Wiley & Sons A/S Background and Objective: In our previous study, we found that flutamide [an androgen receptor (AR) antagonist] inhibited the up-regulation of collagen induced by interleukin (IL)-1β and/or nifedipine in gingival fibroblasts. The present study attempted to verify the role of nitric oxide (NO) in the IL-1β/nifedipine-AR pathway in gingival overgrowth. Material and Methods: Confluent gingival fibroblasts derived from healthy individuals ( n = 4) and those with dihydropyridine-induced gingival overgrowth (DIGO) ( n = 6) were stimulated for 48 h with IL-1β (10 ng/mL), nifedipine (0.34 μ m) or IL-1β + nifedipine. Gene and protein expression were analyzed with real-time RT-PCR and western blot analyses, respectively. Meanwhile, Sircol dye-binding and the Griess reagent were, respectively, used to detect the concentrations of total soluble collagen and nitrite in the medium. Results: IL-1β and nifedipine simultaneously up-regulated the expression of the AR and type-I collagen α1 [ Colα1(I)] genes and the total collagen concentration in DIGO cells ( p < 0.05). IL-1β strongly increased the expression of inducible nitric oxide synthase ( iNOS) mRNA and the nitrite concentration in both healthy and DIGO cells ( p < 0.05). However, co-administration of IL-1β and nifedipine largely abrogated the expression of iNOS mRNA and the nitrite concentration with the same treatment. Spearman's correlation coefficients revealed a positive correlation between the AR and total collagen ( p < 0.001), but they both showed a negative correlation with iNOS expression and the NO concentration ( p < 0.001). The iNOS inhibitor, 1400W, enhanced IL-1β-induced AR expression; furthermore, the NO donor, NONOate, diminished the expression of the AR to a similar extent in gingival fibroblasts derived from both healthy patients and DIGO patients ( p < 0.05). Conclusion: IL-1β-induced NO attenuated AR-mediated collagen production in human gingival fibroblasts. The iNOS/NO system down-regulated the axis of AR/ Colα1(I) mRNA expression and the production of AR/total collagen proteins by DIGO cells.
- Subjects
TAIWAN; FIBROBLASTS; GINGIVAL hyperplasia; NITRIC oxide; PERIODONTITIS; POLYMERASE chain reaction; RESEARCH funding; STATISTICS; T-test (Statistics); U-statistics; WESTERN immunoblotting; DATA analysis; EQUIPMENT &; supplies; CASE-control method; REVERSE transcriptase polymerase chain reaction; DATA analysis software; PHYSIOLOGY
- Publication
Journal of Periodontal Research, 2012, Vol 47, Issue 6, p701
- ISSN
0022-3484
- Publication type
Article
- DOI
10.1111/j.1600-0765.2012.01484.x