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- Title
EFFECT OF BYPRODUCTS FROM THE OZONATION OF PYRENE: BIPHENYL-2,2',6,6'-TETRACARBALDEHYDE AND BIPHENYL-2,2',6,6'-TETRACARBOXYLIC ACID ON GAP JUNCTION INTERCELLULAR COMMUNICATION AND NEUTROPHIL FUNCTION.
- Authors
Luster-Teasley, Stephanie L.; Ganey, Patricia E.; DiOrio, Mary; Ward III, Joseph S.; Maleczka Jr., Robert E.; Trosko, James E.; Masten, Susan J.
- Abstract
In this study, biphenyl-2,2′,6,6′-tetracarbaldehyde, an initial byproduct formed from the ozonation of pyrene, and biphenyl-2,2′,6,6′--tetracarboxylic acid, a subsequent pyrene ozonation byproduct, were evaluated using two toxicology assays to compare the toxicity of ozonation byproducts with that of the parent compound. The first assay measured the potential for the compounds to block gap junctional intercellular communication (GJIC) using the scrape loading/dye transfer technique in normal WB-344 rat liver epithelial cells. The second assay evaluated the ability of the compounds to affect neutrophil function by measuring the production of superoxide in a human cell line (HL-60). Pyrene significantly blocked intercellular communication (ƒ = 0.2-0.5) at 40 µM and complete inhibition of communication (ƒ < 0.2) occurred at 50 µ M. Gap junctional intercellular communication in cells exposed to biphenyl-2,2',6,6'-tetracarbaldehyde reached ƒ < 0.5 at a concentration of 15 µM. At concentrations greater than 20 µM, biphenyl-2,2′,6,6′-tetracarbaldehyde was cytotoxic and the inhibition of GJIC was caused by cell death. Biphenyl-2,2′,6,6′-tetracarboxylic acid was neither cytotoxic nor inhibitory to GJIC at the concentrations tested (10-500 µ M). Exposure to biphenyl-2,2′,6,6′-tetracarbaldehyde resulted in a concentration-dependent decrease in phorbol 12-myristate 13-acetate--stimulated O2- production. Neither exposure to pyrene nor biphenyl-2,2′,6,6′-tetracarboxylic acid caused a significant toxic effect on neutrophil function.
- Subjects
PYRENE; NEUTROPHILS; CARBOXYLIC acids; SUPEROXIDES; BIPHENYL compounds
- Publication
Environmental Toxicology & Chemistry, 2005, Vol 24, Issue 3, p26
- ISSN
0730-7268
- Publication type
Article
- DOI
10.1897/04-679.1