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- Title
XENOBIOTIC AND STEROID BIOTRANSFORMATION ENZYMES IN ATLANTIC SALMON (SALMO SALAR) LIVER TREATED WITH AN ESTROGENIC COMPOUND, 4-NONYLPHENOL.
- Authors
Arukwe, Augustine; Förlin, Lars; Goksøyr, Anders
- Abstract
Hepatic microsomal biotransformation reactions with xenobiotic and steroid substrates have been investigated in 4-nonylphenol (NP; 1, 5, 25, and 125 mg/kg body weight)-treated juvenile Atlantic salmon (Salmo salar), in addition to control and estradiol-17β (5 mg/kg, positive control)-treated fish. Treatment of juvenile salmon with NP caused an initial increase and an apparent dose-dependent decrease in progesterone 6β-, 16α, and 17α-hydroxylase activities in liver microsomes. 7-Ethoxyresorufin O-deethylase and UDP-glucuronosyltransferase activities were also reduced. Plasma levels of estradiol-17β (E2) were lowered 24-43% as a result of NP treatment. Immunochemical analysis of CYP1A, CYP2K-like, and CYP3A-like proteins showed 18%, 47%, and 30% reductions in enzyme-linked immunosorbent assay absorbance levels, respectively, in the groups treated with 125 mg NP/kg fish. The group treated with E2 also showed similar reductions. In summary, the present study has demonstrated variations in steroid hydroxylases, cytochrome P450 isozymes, and conjugating enzyme levels in NP-treated juvenile salmon. These results represent a novel aspect of NP effects not previously demonstrated with an environmental estrogen in any fish species or lower vertebrate.
- Subjects
STEROIDS; PHENOL; ATLANTIC salmon; ESTRADIOL; PROGESTERONE; PROTEINS; ENZYMES
- Publication
Environmental Toxicology & Chemistry, 1997, Vol 16, Issue 12, p2576
- ISSN
0730-7268
- Publication type
Article
- DOI
10.1897/1551-5028(1997)016<2576:XASBEI>2.3.CO;2