We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Synthetic and natural surfactant differentially modulate inflammation after meconium aspiration.
- Authors
Hilgendorff, Anne; Rawer, Daniel; Doerner, Martin; Tutdibi, Erol; Ebsen, Michael; Schmidt, Reinhold; Guenther, Andreas; Gortner, Ludwig; Reiss, Irwin
- Abstract
<bold>Objective: </bold>Meconium aspiration syndrome remains a relevant cause of neonatal respiratory failure and is associated with severe pulmonary changes including surfactant inactivation and pronounced inflammatory changes. The present study investigated the effect of two different surfactant preparations-recombinant surfactant protein C surfactant (rSP-C Surf) and natural bovine surfactant-on pulmonary gas exchange and inflammatory response.<bold>Design and Subjects: </bold>Twenty-three newborn piglets were intubated, mechanically ventilated, received 5 ml/kg 20% sterile meconium for induction of lung injury, and were randomized thereafter for controls ( n=7), rSP-C Surf ( n=8), or natural surfactant ( n=8). Surfactants were given as an intratracheal bolus (75 mg/kg) and animals were further ventilated.<bold>Measurements and Results: </bold>Lung function variables, arterial blood gas samples and lung tissues were obtained. Histological evaluation was performed in right lung tissue using an established score. Cytokine mRNA expression (left lung tissue) was quantified using TaqMan real-time PCR (DeltaDeltaCT method, normalized to controls). In addition to significant improvement in gas exchange and lung function, histological evaluation showed significantly lower sum scores in the rSP-C Surf group than in controls). Cytokine mRNA expression of IL-1beta in whole lung tissue was significantly lower after administration of rSP-C Surf than in natural surfactant and controls whereas IL-10 mRNA expression was significantly induced in both surfactant groups.<bold>Conclusions: </bold>Surfactant administration improved both gas exchange and pulmonary inflammatory cytokine transcription. Mechanisms underlying the differential inflammatory response in both surfactant preparations need to be further addressed.
- Subjects
MECONIUM aspiration syndrome; PEDIATRIC respiratory diseases; SURFACE active agents; BLOOD coagulation factors; PROTEIN C; PNEUMONIA; PIGLETS; LABORATORY swine; RESPIRATORY distress syndrome treatment; PULMONARY surfactant; ANIMAL experimentation; ANIMAL populations; ANIMALS; BIOLOGICAL models; CATTLE; CYTOKINES; POLYMERASE chain reaction; PULMONARY gas exchange; RESPIRATORY distress syndrome; SWINE; DISEASE complications; THERAPEUTICS
- Publication
Intensive Care Medicine, 2003, Vol 29, Issue 12, p2247
- ISSN
0342-4642
- Publication type
journal article
- DOI
10.1007/s00134-003-1984-8